University of South Alabama

Vectors are provided either as DNA or as E.coli cultures at discretion of the Core*. The Core is committed to providing materials to investigators outside of the University of South Alabama for basic research purposes. Investigators outside University of South Alabama are requested to execute a Material Transfer Agreement (MTA). Investigators from institutions, which are signatory to UBMTA (the list of signatories to UBMTA can be found here: http://www.autm.net/Content/NavigationMenu/TechTransfer/TechnologyTransferResources
/UBMTA_Signatories.htm
) may execute UBMTA Implementation Letter. Investigators from institutions not signatories to UBMTA should execute the following MTA.

Plasmids Available

Name

Description (essential features)

Deduced sequence

Map

Reference

pMA2640

Retroviral vector encoding CMV-driven rtTA Advanced (Tet-On) followed by IRES-EGFP-Blasticidin resistance

.doc

[1]

pMA2818

Retroviral vector encoding LTR-driven tTA Advanced (Tet-Off) followed by IRES-EGFP-Blasticidin resistance

.doc .doc

Unpublished**

pMA2641

Retroviral vector encoding LTR-driven rtTA Advanced (Tet-On) followed by IRES-EGFP-Blasticidin resistance

.doc .doc

[1]

pMA2706

Lentivirus vector encoding Doxycycline-controlled Gaussia (secreted) luciferase and resistance to puromycin.

.doc .doc

[1]

pMA2751

Lentivirus vector encoding Doxycycline-controlled DsRed Express and resistance to puromycin.

.doc .doc

[1]

pMA2780

Lentivirus vector with doxycycline-regulated promoter, polylinker, and puromycin resistance gene

.doc .doc

[1]

pMA2867

Lentivirus vector for doxycycline-regulated gene knockdown. shRNA is co-expressed with mCherry from doxycycline-regulated promoter

.doc .doc

[1]

pMA1870

Retroviral construct (MoMuLV) encoding a fusion between 166 N-terminal aa of the rat PDE4D4 and EGFP

.doc .doc

[2]

pMA1629

Retroviral (MoMuLV) vector, puromycin resistance, extensive polylinker

.doc .doc

[3]

pMA1629rc

Retroviral (MoMuLV) vector, puromycin resistance, extensive polylinker

.doc .doc

Unpublished**

pMA1662

Retroviral (MoMuLV) vector, hygromycin resistance, extensive polylinker

.doc .doc

Unpublished**

pMA1662rc

Retroviral (MoMuLV) vector, hygromycin resistance, extensive polylinker

.doc .doc

Unpublished**

pMA2279

Retroviral vector  (MoMuLV) encoding CMV-driven AcGFP-b-Actin (human) fusion

.doc .doc

[3]

pMA2280

Retroviral vector  (MoMuLV) encoding CMV-driven AcGFP-Tubulin (human) fusion

.doc .doc

[3]

pMA2508

Retroviral vector  (MoMuLV) encoding EGFP-PDE5A1 fusion

.doc .doc

[4]

pMA2340 (1-6)

Retroviral vectors encoding shRNAs to rat PDE5A1

.doc .doc

[4]

pMA2462

Vector for tetracycline-regulated expression of hTFAM

.doc .doc

[5]

pMA1804

Vector encoding CMV-driven nuclearly-localized EGFP fusion

.doc .doc

[5]

pMA1817

Vector encoding CMV-driven nuclearly-localized EGFP fusion

.doc .doc

[5]

pMA1696

Retroviral vector encoding CMV-driven HA-tagged rat NAP1

.doc .doc

[6]

pMA2181

Retroviral vector for shRNA-mediated knockdown of the rat NAP1

.doc .doc

[6]

pMA2182

Retroviral vector for shRNA-mediated knockdown of the rat NAP1

.doc .doc

[6]

pMA2183

Retroviral vector for shRNA-mediated knockdown of the rat NAP1

.doc .doc

[6]

pMA2184

Retroviral vector for shRNA-mediated knockdown of the rat NAP1

.doc .doc

[6]

pMA2239

A cassette encoding mCherry

.doc .doc

[7]

pMA2227

CMV-driven, mitochondrially targeted DsRed Express

.doc .doc

[7]

pMA1261

CMV-driven, mitochondrially targeted EGFP

.doc .doc

Unpublished**

pMA2008

Vector for generation of stable transfectants, G418 resistance

.doc .doc

Unpublished**

pMA2008HA

Vector for generation of stable transfectants, G418 resistance, HA-tag

.doc .doc

Unpublished**

pMA2008Myc

Vector for generation of stable transfectants, G418 resistance, Myc-tag

.doc .doc

Unpublished**

* Investigators outside University of South Alabama are requested to provide their FedEx account numbers for shipping.
** Unpublished vectors are provided on a collaborative basis. Please contact the Core's Director for details (e-mail: malexeye@jaguar1.usouthal.edu).

  1. Alexeyev, M.F., R. Fayzulin, I.N. Shokolenko, and V. Pastukh, A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity. Mol Biol Rep, 2010. 37(4): p. 1987-91.
  2. Creighton, J., B. Zhu, M. Alexeyev, and T. Stevens, Spectrin-anchored phosphodiesterase 4D4 restricts cAMP from disrupting microtubules and inducing endothelial cell gap formation. J Cell Sci, 2008. 121(Pt 1): p. 110-9.
  3. Prasain, N., M. Alexeyev, R. Balczon, and T. Stevens, Soluble adenylyl cyclase-dependent microtubule disassembly reveals a novel mechanism of endothelial cell retraction. Am J Physiol Lung Cell Mol Physiol, 2009. 297(1): p. L73-83.
  4. Zhu, B., L. Zhang, M. Alexeyev, D.F. Alvarez, S.J. Strada, and T. Stevens, Type 5 phosphodiesterase expression is a critical determinant of the endothelial cell angiogenic phenotype. Am J Physiol Lung Cell Mol Physiol, 2009. 296(2): p. L220-8.
  5. Pastukh, V., I. Shokolenko, B. Wang, G. Wilson, and M. Alexeyev, Human mitochondrial transcription factor A possesses multiple subcellular targeting signals. FEBS J, 2007. 274(24): p. 6488-99.
  6. Clark, J., D.F. Alvarez, M.F. Alexeyev, J.A. King, L. Huang, M.C. Yoder, and T. Stevens, Regulatory role for nucleosome assembly protein-1 in the proliferative and vasculogenic phenotype of pulmonary endothelium. Am J Physiol Lung Cell Mol Physiol, 2007.
  7. Pastukh, V., I.N. Shokolenko, G.L. Wilson, and M.F. Alexeyev, Mutations in the passenger polypeptide can affect its partitioning between mitochondria and cytoplasm : Mutations can impair the mitochondrial import of DsRed. Mol Biol Rep, 2008. 35(2): p. 215-23.
 

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