Naga S. Annamdevula

Graduate Student, Year 6
Department of Chemical and Biomolecular Engineering
College of Engineering, University of South Alabama
Department of Pharmacology and the Center for Lung Biology
College of Medicine, University of South Alabama
Silas J. Leavesley, Ph.D.
Department of Chemical and Biomolecular Engineering and Center for Lung Biology

I am interested in studying  the role of different phosphodiesterase (PDE) isoforms (PDE4A, B, and D) in regulating cAMP compartmentalization and maintaining barrier permeability in pulmonary microvascular endothelial cells. Recently, fret reporters have been utilized to measure cAMP signals in cells. However, measuring cAMP signals in living cells have been difficult using current FRET imaging techniques. Thus, I’m utilizing hyperspectral FRET microscopy and image cytometry analysis to measure subcellular location of cAMP signals and study how these signals are regulated by PDEs. Our studies will offer an effective approach to measure small second messenger signals in live cells with improved signal to noise ratio.

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