Natalie R. Gassman, Ph.D.

Assistant Professor of Oncologic Sciences

2016-03-09 MCI Natalie Gassman resized.jpg

 

USA Mitchell Cancer Institute
1660 Springhill Ave.
Mobile, Ala., 36604
(251) 445-8430
nrgassman@health.southalabama.edu 

 

Professional Profile

Education:
- Ph.D. in Physical Chemistry, University of California at Los Angeles, Los Angeles, CA, 2001-2008
- B.A. in Chemistry, Michigan State University, East Lansing, MI, 1997-2001

Professional Appointments:
- Assistant Professor of Oncologic Sciences, USA Mitchell Cancer Institute (2015-present) 
- Assistant Professor of Pharmacology, USA College of Medicine (2015-present) 
- Member of Editorial Board, Cancer and Oncology Research (2013-present)
- New Investigator Chair, Environmental Genomics and Mutagenesis Society (2017)

Research Experience:
- University of South Alabama Mitchell Cancer Institute, Mobile, AL, Molecular & Metabolic Oncology, Assistant Professor of Oncologic Sciences, July 2015 to present

  • Examining the influence of environmental endocrine disruptors on DNA damage signaling and repair
  • Developing advanced fluorescent microscopy methodologies for examining the recruitment of DNA repair proteins to the sites of DNA damage

- National Institute of Environmental Health Sciences. Research Triangle Park, NC, Genome Integrity and Structural Biology Laboratory, Postdoctoral Fellow with Dr. Samuel H. Wilson, Oct. 2010 to June 2015

  • Project 1: DNA polymerase β structural-function studies with single molecule fluorescence techniques
  • Project 2: Role of PARP-1 hyperactivation in base excision repair deficient cell lines
  • Project 3: Bisphenol A suppresses base excision repair triggered by environmental genotoxic stressors

- Wake Forest University School of Medicine, Winston Salem, NC, Cancer Biology, Postdoctoral Fellow with Dr. Karin D. Scarpinato, July 2009-Oct. 2010

  • Project: Cellular distribution, assembly and DNA binding of human mismatch repair proteins MSH2 and MSH6

- Wake Forest University, Winston Salem, NC, Physics, Postdoctoral Fellow with Dr. Jed Macosko, July 2008-July 2009

  • Project 1: Drug Discovery at the Nanoscopic Level: Lab-on-Bead processing of nucleic acid encoded chemical libraries
  • Project 2: Cellular distribution, assembly and DNA binding of human mismatch repair proteins MSH2 and MSH6 (In collaboration with Karin Scarpinato)

- University of California, Los Angeles, Los Angeles, CA, Chemistry and Biochemistry, Graduate Student with Dr. Shimon Weiss, September 2002-July 2008

  • Thesis Project: Single-molecule characterization of protein-protein interactions and transcription mechanisms of Shewanella oneidensis RNA polymerase
  • Project: Mapping of transcription factor binding sites with combined atomic force and fluorescence microscopy

Selected Peer-reviewed Publications:
1. Ebenstein Y, Gassman N, Kim S, Antelman J, Kim Y, Ho S, Samuel R, Michalet X, Weiss S. Lighting up individual DNA binding proteins with quantum dots. Nano Lett.2009 Apr;9(4):1598-603. doi: 10.1021/nl803820b. PubMed PMID: 19290670; PubMed Central PMCID: PMC3084662.

2. Gassman NR, Ho SO, Korlann Y, Chiang J, Wu Y, Perry LJ, Kim Y, Weiss S. In vivo assembly and single-molecule characterization of the transcription machinery from Shewanella oneidensis MR-1. Protein Expr Purif. 2009 May;65(1):66-76. doi: 10.1016/j.pep.2008.11.013. Epub 2008 Dec 10. PubMed PMID: 19111618; PubMed Central PMCID: PMC3084661.

3. Ebenstein Y, Gassman N, Kim S, Weiss S. Combining atomic force and fluorescence microscopy for analysis of quantum-dot labeled protein-DNA complexes. J Mol Recognit. 2009 Sep-Oct;22(5):397-402. doi: 10.1002/jmr.956. PubMed PMID: 19452448; PubMed Central PMCID: PMC3085854.

4. Gassman NR, Nelli JP, Dutta S, Kuhn A, Bonin K, Pianowski Z, Winssinger N, Guthold M, Macosko JC. Selection of bead-displayed, PNA-encoded chemicals. J Mol Recognit. 2010 Sep-Oct;23(5):414-22. doi: 10.1002/jmr.1007. PubMed PMID: 19957300; PubMed Central PMCID: PMC3016959.

5. Gassman NR, Clodfelter JE, McCauley AK, Bonin K, Salsbury FR Jr, Scarpinato KD. Cooperative nuclear localization sequences lend a novel role to the N-terminal region of MSH6. PLoS One. 2011 Mar 17;6(3):e17907. doi: 10.1371/journal.pone.0017907. PubMed PMID: 21437237; PubMed Central PMCID: PMC3060103.

6. Gassman NR, Stefanick DF, Kedar PS, Horton JK, Wilson SH. Hyperactivation of PARP triggers nonhomologous end-joining in repair-deficient mouse fibroblasts. PLoS One. 2012;7(11):e49301. doi: 10.1371/journal.pone.0049301. Epub 2012 Nov 7. PubMed PMID: 23145148; PubMed Central PMCID: PMC3492265.

7. Masaoka A, Gassman NR, Kedar PS, Prasad R, Hou EW, Horton JK, Bustin M, Wilson SH. HMGN1 protein regulates poly(ADP-ribose) polymerase-1 (PARP-1) self-PARylation in mouse fibroblasts. J Biol Chem. 2012 Aug 10;287(33):27648-58. doi: 10.1074/jbc.M112.370759. Epub 2012 Jun 26. PubMed PMID: 22736760; PubMed Central PMCID: PMC3431713.

8. Masaoka A, Gassman NR, Horton JK, Kedar PS, Witt KL, Hobbs CA, Kissling GE, Tano K, Asagoshi K, Wilson SH. Interaction between DNA Polymerase β and BRCA1. PLoS One. 2013 Jun 27;8(6):e66801. Print 2013. PubMed PMID: 23826138; PubMed Central PMCID: PMC3694962.

9. Horton JK, Stefanick DF, Gassman NR, Williams JG, Gabel SA, Cuneo MJ, Prasad R, Kedar PS, Derose EF, Hou EW, London RE, Wilson SH. Preventing oxidation of cellular XRCC1 affects PARP-mediated DNA damage responses. DNA Repair (Amst). 2013 Sep;12(9):774-85. doi: 10.1016/j.dnarep.2013.06.004. Epub 2013 Jul 18. PubMed PMID: 23871146; PubMed Central PMCID: PMC3924596.

10. Levy-Sakin M, Grunwald A, Kim S, Gassman NR, Gottfried A, Antelman J, Kim Y, Ho SO, Samuel R, Michalet X, Lin RR, Dertinger T, Kim AS, Chung S, Colyer RA, Weinhold E, Weiss S, Ebenstein Y. Toward single-molecule optical mapping of the epigenome. ACS Nano. 2014 Jan 28;8(1):14-26. doi: 10.1021/nn4050694. Epub 2013 Dec 20. PubMed PMID: 24328256; PubMed Central PMCID:  MC4022788.

11. Prasad R, Horton JK, Chastain PD 2nd, Gassman NR, Freudenthal BD, Hou EW, Wilson SH. Suicidal cross-linking of PARP-1 to AP site intermediates in cells undergoing base excision repair. Nucleic Acids Res. 2014 Jun;42(10):6337-51. doi: 10.1093/nar/gku288. Epub 2014 Apr 25. PubMed PMID: 24771347; PubMed Central PMCID: PMC4041460.

12. Horton JK, Stefanick DF, Prasad R, Gassman NR, Kedar PS, Wilson SH. Base excision repair defects invoke hypersensitivity to PARP inhibition. Mol Cancer Res. 2014 Aug;12(8):1128-39. doi: 10.1158/1541-7786.MCR-13-0502. Epub 2014 Apr 25. PubMed PMID: 24770870; PubMed Central PMCID: PMC4135006.

13. Gassman NR, Coskun E, Stefanick DF, Horton JK, Jaruga P, Dizdaroglu M, Wilson SH. Bisphenol a promotes cell survival following oxidative DNA damage in mouse fibroblasts. PLoS One. 2015 Feb 18;10(2):e0118819. doi: 10.1371/journal.pone.0118819. eCollection 2015. PubMed PMID: 25693136; PubMed Central PMCID: PMC4334494.

14. Horton JK, Gassman NR, Dunigan BD, Stefanick DF, Wilson SH. DNA polymerase β- dependent cell survival independent of XRCC1 expression. DNA Repair (Amst). 2015 Feb;26:23-9. doi: 10.1016/j.dnarep.2014.11.008. Epub 2014 Dec 3. PubMed PMID: 25541391; PubMed Central PMCID: PMC4308486.

15. Gassman NR, Wilson SH. Micro-irradiation tools to visualize base excision repair and single-strand break repair. DNA Repair (Amst). 2015 Jul;31:52-63. doi: 10.1016/j.dnarep.2015.05.001. Epub 2015 May 5. Review. PubMed PMID: 25996408; PubMed Central PMCID: PMC4458156.

16. Kirby TW, Gassman NR, Smith CE, Pedersen LC, Gabel SA, Sobhany M, Wilson SH, London RE. Nuclear localization of the DNA repair scaffold XRCC1: uncovering the functional role of a bipartite NLS. Sci Rep. 2015 Aug 25;5:13405. doi: 10.1038/srep13405. PubMed PMID: 26304019; PubMed Central PMCID: PMC4548243.

17. Gassman N.R., Coskun E., Jaruga P., Dizdaroglu M., Wilson S.H. (2016) Combined effects of high-dose bisphenol A and oxidizing agent (KBrO3) on cellular microenvironment, gene expression, and chromatin structure of Ku70-deficient mouse embryonic fibroblasts. Accepted Environ Health Perspect.

Book Chapters
1. Gassman N.R. (2006). Creating New Opportunities for Improving Existing Therapeutics: Nano-Enabled Drug Delivery Systems Trends Toward 2020. In The Global Technology Revolution: Bio/Nano/Materials Trends and Their Synergies with Information Technology by 2020.

2. Ebenstein, Y.; Gassman, N.R.; Weiss, S. (2010). Mapping Transcription Factors on Extended DNA: A Single Molecule Approach. In A. Graslund, R Rigler, & Jerker Widengren (Eds.), Single Molecule Spectroscopy in Chemistry, Physics and Biology (203-216). Springer.

3. Gassman N.R. and Wilson S.H. (submitted). Bisphenol A and Nongenotoxic Drivers of Cancer. In Translational Toxicology and Therapeutics: Windows of Developmental Susceptibility in Reproduction and Cancer. Wiley Global Research 

 

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