Flow Cytometry and Imaging Core Laboratories

Provides researchers with analytical cytometry and cell sorting services, as well as the scientific expertise necessary to integrate this technology into research projects. The core maintains two analysis instruments that are intended to be user-operated after appropriate training from the core manager: 1) BD FACS Canto II, a three laser/eight color flow cytometer and 2) Celigo Imaging Cytometer, a microplate based system for analysis of adherent cells, imaged in brightfields, blue, green and red fluorescent channels. High speed cell sorting services are provided by the core manager using a four laser/14 color BD SORP Aria II cell sorter capable of up to four-way collection and single cell deposition for cloning or single cell PCR. The core manager is available for consultation in experimental design and education of the staff for both instrument operation and sample preparation.


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