Postdoctoral Studies: Texas Heart Institute
Ph.D. - National Academy of Sciences of Ukraine, Kiev
Mitochondrial DNA Maintenance, Damage, Repair, Degradation and Mutagenesis. Mitochondria as Targets for Gene Therapy.
Mitochondria are central players in processes of cell life and death. Mitochondrial dysfunction has been linked to such diverse conditions as mitochondrial diseases, cardiovascular disease, cancer, diabetes, and aging. Research in this lab is directed towards understanding the mechanisms for the maintenance of mtDNA integrity, developing mouse models for mitochondrial diseases caused by mutations in mtDNA as well as devising gene therapy strategies for mitochondrial disorders.
Shokolenko, I.N., M.F. Alexeyev, S.P. LeDoux, and G.L. Wilson, The approaches for manipulating mitochondrial proteome. Environ Mol Mutagen, 2010. 51(5): p. 451-61.
Alexeyev MF. Is there more to aging than mitochondrial DNA and reactive oxygen species? FEBS J. 2009 Oct;276(20):5768-87.
Shokolenko I, Venediktova N, Bochkareva A, Wilson GL, Alexeyev MF. Oxidative stress induces degradation of mitochondrial DNA. Nucleic Acids Res. 2009 37: 2539-2548
Pastukh V, Shokolenko IN, Wilson GL, Alexeyev MF. Mutations in the passenger polypeptide can affect its partitioning between mitochondria and cytoplasm : Mutations can impair the mitochondrial import of DsRed. Mol Biol Rep. 2008 Jun;35(2):215-23.
Alexeyev MF, Venediktova N, Pastukh V, Shokolenko I, Bonilla G, Wilson GL. Selective elimination of mutant mitochondrial genomes as therapeutic strategy for the treatment of NARP and MILS syndromes. Gene Ther. 2008 Apr;15(7):516-23.
Pastukh V, Shokolenko I, Wang B, Wilson G, Alexeyev M. Human mitochondrial transcription factor A possesses multiple subcellular targeting signals. FEBS J. 2007 Dec;274(24):6488-99.
Alexeyev MF, Ledoux SP, Wilson GL Mitochondrial DNA and aging. In.P. Michael Conn ed. Handbook of models for human aging. Elsevier, 2006
Ruchko M, Gorodnya O, Ledoux SP, Alexeyev MF, Al-Mehdi AB, Gillespie MN. Mitochondrial DNA damage triggers mitochondrial dysfunction and apoptosis in oxidant-challenged lung endothelial cells. Am J Physiol Lung Cell Mol Physiol.288:L530-5 (2005).
Shokolenko IN, Alexeyev MF, LeDoux SP, Wilson GL. TAT-mediated protein transduction and targeted delivery of fusion proteins into mitochondria of breast cancer cells. DNA Repair (Amst). 4:511-8 (2005).
Alexeyev MF, LeDoux SP, Wilson GL Mitochondrial DNA and aging Clin Sci (Lond). 107:355-64 (2004)
Rachek L, Grishko V, Alexeyev M, Pastukh V, LeDoux S, Wilson GL Endonuclease III and endonuclease VIII conditionally targeted into mitochondria enhance mitochondrial DNA repair and cell survival following oxidative stress Nucleic Acids Res.32:3240-7 (2004).
Shokolenko IN, Alexeyev MF, Robertson FM, LeDoux SP, Wilson GL. The expression of exonucleaseIII from E. coli in mitochondria of breast cancer cells diminishes mitochondrial DNA repair capacity and cell survival after oxidative stress. DNA Repair (Amst) 2:471-82 (2003).
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