Introduction to Research Methods - IDL 577 (3 credits)

Fall Semester, 2010 

Course Director:

Richard Honkanen, Ph.D.    Phone: 460-6859    rhonkanen@jaguar1.usouthal.edu 

Office MSB 2362

Objective: The goal of this course is to give students entering the graduate program didactic training in basic skills utilized in the basic medical science laboratory.  In addition, regulatory issues in biomedical research will be discussed, such as occupational and environmental safety, use of animals and humans in research, and students will complete the appropriate certifications.  The course will be organized in the form of several sequential, functional protocols. The emphasis of the lectures will be the theory upon which the methods are based.

Course hours: The class will meet 2 times per week.  Didactic lectures on theory of techniques and discussions of the regulatory issues will be interwoven. 

Attendance: Required; due to the nature of this course, make-up sessions will not be available

Meeting time: Tuesday 1-2:30 pm Thursday     1-2:30 pm

Grading: Grades will be based on examinations (90%), and participation (10%). Examinations will be distributed the days indicated (see schedule).

Content: An outline of the course is shown below.  Reading assignments: Albert's Molecular Biology of the Cell, 4th Ed., Chapters 8 and 9. Other materials and/or reading assignments may be distributed by individual course faculty.


IDL 577 Introduction to Research Methods

 

 

Week

 

Day  

 

Date

 

Focus

 

Instructor

 

Room (MSB)

1

Tues

8/24

Introduction/ Radiation Safety Part 1

Honkanen /Wiik

2360

1

Thurs

8/26

Radiation Safety Part II

Wiik

2360

2

Tues

8/31

Radiation Safety Part III

Wiik

2360

2

Thurs

9/2

Environmental Safety

Malone

2360

3

Tues

9/7

Biosafety, Occupational Health Programs

Henry

2360

3

Thurs

9/9

Library Database Searching

Clanton

 

BML Computer Lab

 

4

 

Tues

 

9/14

 

IACUC; Animal Use Rodent Handling, Anesthesia

 

Schuler

 

2360

 

 

4

 

Thurs

 

9/16

 

Research Grants and Tech Transfer

Turbeville/Taylor

2360

5

Tues

9/21

 

Basic Laboratory Skills

 

Balczon

 

2360

 

5

 

Thurs

 

9/23

Approach to experimental design

Rocic

2360

 

6

 

Tues

 

9/28

 

Buffer Preparation, pH Measurement, Spectrophotometry

 

Ayling

 

2360

 

6

Thurs

 

9/30

 

PCR Therapy and Applications

 

Honkanen

 

2360

 

7

 

Tues

 

10/5

 

FALL BREAK

 

 

 

7

 

 

Thurs

 

10/7

 

Isolation of Total and PolyA RNA

Gene Expression Analysis

 

Alexeyev

 

2360

 

8

 

Tues

10/12

Principles of Bacterial Culture and Plasmids

Audia* Class immediately after IDL580

2080

 

8

 

Thurs

 

10/14

 

Bacterial Culture: Aseptic Technique Impact of Antibiotics & Serial Dilution Restriction Digestion of PCR Products

Plasmid Extraction Ligations Cloning & Blue/White Screening Plasmid Transformation by Plasmid Extraction of Cloned DNA CaC12

 

Audia*  Class will meet immediately after IDL 580

 

2080

9

 

Tues

 

10/19

Phage mutagen

Audia*

2080

 

9

 

Thurs

 

10/21

 

EXAM #1   (Lectures 9/21- 10/19)

 

Honkanen

 

2360

 

Week

 

Day  

 

Date

 

Focus

 

Instructor

 

Room (MSB)

 

10

 

Tues

 

10/26

 

Protein expression systems

Honkanen

2360

 

10

 

Thurs

 

10/28

Protein purification/Column Chromatography

Brewer

2080

 

11

Tues

 

11/2

Protein Electrophoresis and Electro-Blotting 

Gerthoffer

2360

 

11

 

Thurs

 

11/4

 

Western-Blot

 

Gerthoffer

 

2360

 

12

 

Tues

 

11/9

 

ELISA, Flow Cytometry

 

Barrington

 

2360

 

12

Thurs

 

11/11

 

2D Electrophoresis, proteomics and genomics

 

Foster

2080

 

13

Tues

 

11/16

 

Exam #2 (Lecture 10/26- 11/11)

 

 

 

2360

 

13

 

Thurs

 

11/18

 

Mass Spectrometry I       

 

Pannell

 

2360

 

14

 

Tues

 

11/23

 

Mass Spectrometry II      

 

Pannell

 

2360

 

14

 

Thurs

 

11/25

Thanksgiving Holiday

 

 

 

15

 

Tues

 

11/30

 

Basic Cell Culture Methods (Sterile Technique, Media  Prep, Cell Passage)

 

Alvarez

2360

 

15

 

Thurs

 

12/2

Cell Viability Cell Counting

Alvarez

2360

 

16

 

Tues

 

12/7

 

Cell Transfection/Gene Delivery

 

Alexeyev

 

2360

 

16

 

Thurs

 

12/9

Fluorescence Techniques/Theory TG Fluorescence Microscopy-Evaluation of Gene Delivery

 

Al-Mehdi

 

2360/3140

 

17

 

Mon

 

12/13

 

EXAM #3  (Lectures 11/18-12/9)

 

Honkanen

 

2360

 

 

The goal of this course is to give students entering the graduate program didactic training in basic skills utilized in the basic medical science laboratory.  In addition, regulatory issues in biomedical research will be discussed, such as occupational and environmental safety, use of animals and humans in research, and students will complete the appropriate certifications.  The course will be organized in the form of several sequential, functional protocols. The emphasis of the lectures will be the theory upon which the methods are based.

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