Postdoctoral Studies: Harvard Medical School and University of Vermont
Ph.D. - University of Illinois
DNA damage and repair, particularly mitochondrial DNA and how these processes play roles in the etiologies of diabetes, neurodegenerative diseases, cancer, and the normal process of aging. We utilize molecular biology technology study the damage and repair in specific sequences of both nuclear and mitochondrial DNA. Our particular interests lie in understanding how specific lesions are repaired and factors that may prevent this repair and cause disease processes.
Rachek LI, Thornley NP, Grishko VI, LeDoux SP, Wilson GL. Protection of INS-1 cells from free fatty acid-induced apoptosis by targeting hOGG1 to mitochondria. Diabetes 55:1022-1028 (2006).
Rachek LI, Grishko VI, LeDoux SP, Wilson GL. Role of nitric oxide-induced mtDNA damage in mitochondrial dysfunction and apoptosis. Free Radic. Biol. Med. 40:754-762 (2006).
Gillespie MN, Ziel KA, Grishko V, Campbell CC, Wilson GL. Genomic threats from physiological signals in lung vascular cells. Chest 128:611S-612S (2005).
Harrison JF, Hollensworth SB, Spitz Dr, Copeland WC, Wilson GL, LeDoux SP. Oxidative stress-induced apoptosis in neurons correlates with mitochondrial DNA base excision repair pathway imbalance. Nucleic Acids Res. 33(14):4660-4671 (2005).
Druzhyna NM, Musiyenko SI, Wilson GL, LeDoux SP. Cytokines induce nitric oxide-mediated mtDNA damage and apoptosis in oligodendrocytes. Protective role of targeting 8-oxoguanine glycosylase to mitochondria. J. Biol. Chem. 280(22):21673-21679 (2005).
Ziel KA, Grishko V, Campbell CC, Breit JF, Wilson GL, Gillespie MN. Oxidants in signal transduction: Impact on DNA integrity and gene expression. FASEB J. 19(3):387-394 (2005).
Grishko V, Rachek L, Musiyenko S, LeDoux SP, Wilson GL. Involvement of mtDNA damage in free fatty acid-induced apoptosis. Free Radic Biol Med. 38(6):755-762 (2005).
Shokolenko IN, Alexeyev MF, LeDoux SP, Wilson GL. TAT-mediated protein transduction and targeted delivery of fusion proteins into mitochondria of breast cancer cells. DNA Repair (Amst). 4(4):511-518 (2005).
Grishko VI, Rachek LI, Spitz DR, Wilson GL, LeDoux SP. Contribution of mitochondrial DNA repair to cell resistance from oxidative stress. J Biol Chem. 280(10):8901-8905 (2005).
Druzhyna, NM, Hollensworth, SB, Kelley, MR, Wilson, GL, and Ledoux, SP. Targeting human 8-oxoguanine glysosylase to mitochondria of oligodendrocytes protects against menadione-induced oxidative stress. Glia 42:370-378 (2003).
Bozner, P, Druzhyna, NM, Bryant-Thomas, TK, LeDoux, SP, Wilson, GL, and Pappolla, MA. Deficiency of chaperonin 60 in Down's syndrome. J Alzheimer's Dis. 4:479-486 (2002).
Shokolenko, IN, Alexeyev, MF, Robertson, FM, LeDoux, SP, and Wilson, GL. The expression of Exonuclease III from E. coli in mitochondria of breast cancer cells diminishes mitochondrial repair capacity and cell survival after oxidative stress. DNA Repair (Amst.) 2:471-482 (2003).
Dobson, AW, Grishko, V, LeDoux, SP, Kelley, MR, Wilson, GL, and Gillespie, MN. Enhanced mtDNA repair capacity protects pulmonary artery endothelial cells from oxidant-mediated death. Am J Physiol Lung Cell Mol Physiol. 283:L205-210 (2002).
Dobson, AW, Kelley MR, Wilson, GL, and LeDoux, SP. Targeting DNA repair proteins to mitochondria. Methods Mol Biol. 197:351-362 (2002).
Rachek, LI, Oberyszn, TM, D'Ambrosio, SM, Saavedra, JE, Keefer, LK, LeDoux, and Wilson, GL. Conditional targeting of the DNA repair enzyme hOGG1 into mitchondria. J Biol Chem. 277:44932-44937 (2002).
Shokolenko, I, Oberyszyn, T.M., D'Ambrosio, S.M., Saavedra, J.E., Keefer, L.K., LeDoux, S.P., Wilson, G.L., and Robertson, F.M. Protection of human keratinocyte mtDNA by low-level nitric oxide. Nitric Oxide 5:555-60 (2001).
Grishko, V., Solomon, M., Breit, J.F., Killilea, D.W., LeDoux, S.P., Wilson, G.L., and Gillespie, M.N. Hypoxia promotes oxidative base modifications in the pulmonary artery endothelial cell VEGF gene. FASEB Journal 15:1267-1269 (2001).
Grishko, V., Solomon, M., Wilson, G.L., LeDoux, S.P., and Gillespie, M.N. Oxygen radical-induced mitochondrial DNA damage and repair in pulmonary vascular endothelial cell phenotypes. Am J Physiol. Lung Cell Mol Physiol. 280:L1300-L1308 (2001).
Wang, G., Chen, Z., Zhang, S., Wilson, G.L., and Jing, K. Detection and determination of oligonucleotide triplex formation-mediated transcription-coupled DNA repair in HeLa nuclear extracts. Nucleic Acids Res. 29:1801-1807 (2001).
Dobson, A.W., Xu, Y., LeDoux, S.P., and Wilson, G.L. Enhanced mtDNA repair and cellular survival following oxidative stress by targeting the hOGG repair enzyme to mitochondria. J Biol Chem. 275:37518-37523 (2000).
Druzhyna, N., Smulson, M.E., LeDoux, S.P., and Wilson G.L. Poly (ADP-ribose) polymerase facilitates the repair of N-methylpurines in mtDNA. Diabetes 49:1849-1855 (2000).
Hollensworth B.S., Shen C., Sim, J.E., Spitz, D.R., Wilson, G.L., and LeDoux, S.P. Glial cell type specific responses to menadione-induced oxidative stress. Free Radical Biology and Medicine 28:161-174 (2000).
Pappolla, M.A., Chyan Y.J., Poeggler, B., Frangione B., Wilson, G.L., Ghiso, J., and Reiter R.J. An assessment of the antioxidant and the antiamyloidogenic properties of melatonin: implications for Alzheimer's disease. J of Neural Transmisson 107:203-231 (2000).
Grishko, V.I., Druzhyna, N., LeDoux, S.P., and Wilson, G.L. Nitric oxide-induced damage to mtDNA and its subsequent repair. Nucleic Acids Res. 27:4510-4516 (1999).
Pappolla, M.A., Chyan, Y.J., Poeggeler, B. Bozner, P., Ghiso, J., LeDoux, S.P., and Wilson, G.L. Alzheimer protein mediated oxidative damage of mitochondrial DNA: Prevention by melatonin. J Pineal Res. 27:226-229 (1999).
LeDoux, S.P., Driggers W.J., Hollensworth, B.S., and Wilson, G.L. Repair of alkylation and oxidative damage in mitochondrial DNA. Mutat Res. 434:149-159 (1999).
Druzhyna, N., Nair, R.G., LeDoux, S.P., and Wilson, G.L. Defective repair of oxidative damage in mitochondrial DNA in Down's syndrome. Mutat Res. 409:81-89 (1998).
LeDoux, S.P., Shen, C., Grishko, V.I., Fields, P.A., Gard, A.L., and Wilson, G.L. Glial cell-specific differences in response to alkylation damage. Glia 24:304-312 (1998).
Gillespie, M.N., Killilea, D.W., Solomon, M., Babal, P., LeDoux, S.P., and Wilson, G.L. Hypoxia causes oxidant lesions in the rat pulmonary artery smooth muscle cell VEGF gene; Potential link to VEGF mRNA expression. Chest 114:45 (1998).
Bozner, P., Grishko, V., LeDoux, S.P., Wilson, G.L., and Pappolla, M.A. The amyloid -protein induces oxidative damage of mitochondrial DNA. J Neuropathol Exp Neurol. 56:1356-1362 (1997).
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